<p>Lecture, three hours; laboratory, two hours. Requisites: Life Sciences 7A, 7B, 7C, 23L. Over last two decades, there has been explosion of new techniques in light microscopy which has provided us with invaluable tools for biological research. Study of light microscopy techniques currently used in research laboratories. Basics of light microscopy (image formation, magnification, resolution, contrast), widefield and fluorescence microscopy, optical sections (confocal, multi-photon, light-sheet and total internal reflection fluorescence microscope), and super-resolution microscopy. Laboratory sessions include setting up and using simple, rail-based microscope; hands-on time and demonstrations on brightfield/epiflurorescence, confocal, light-sheet and super-resolution microscopes. Letter grading.</p>

Molecular, Cell, and Developmental Biology

Combining rigorous, inclusive pedagogy with opportunities for co-curricular activities—including research, internships, and community engagement—the College provides students with diverse learning experiences that helps them thrive, become critical thinkers, and transform society.

College of Letters and Science

The major combines essential background studies in mathematics, chemistry, and physics with a general introduction to all of the biological subjects, as well as in-depth exposure to key topics in molecular, cell, and developmental biology.

General Catalog

Principles of Light Microscopy

MCDBIO160

4 units